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Materials. Synthetic cDNA corresponding to IFNA2 was obtained from Operon (Huntsville, Alabama), and was cloned into the pET-SUMO expression vector 25, 26 (a generous gift from Christopher Lima, Sloan-Kettering Institute). Protein was expressed in Escherichia coli BL21(DE3) cells, and the soluble protein was purified using a Nickel Sepharose 6 Fast Flow column (GE Healthcare Life Sciences

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